MiSeq System Sequencing Platforms
Cluster Generation and Sequencing
MiSeq Reagent Kit v2 MiSeq Reagent Kit v3 Read Length 1 × 36 bp 2 × 25 bp 2 × 150 bp 2 × 250 bp 2 × 75 bp 2 × 300 bp Total Time* ~4 hrs ~5.5 hrs ~24 hrs ~39 hrs ~21 hrs ~56 hrs Output 540–610 Mb 750–850 Mb 4.5–5.1 Gb 7.5–8.5 Gb 3.3–3.8 Gb 13.2–15 Gb MiSeq Reagent Kit v2 Micro MiSeq Reagent Kit v2 Nano Read Length 2 × 150 bp 2 × 250 bp 2 × 150 bp Total Time* ~19 hrs ~28 hrs ~17 hrs Output 1.2 Gb 500 Mb 300 Mb List number: - R
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meaning of list number: - “r” means refurbished, “po” means preowned, “u” means used, “t” means trading, “m” means own manufactured, “ad” means authorised dealer of original equipment manufacturer.